Effects of autologous human serum on culturing primary osteoblast cells
نویسندگان
چکیده
منابع مشابه
The Effects of Iron Oxide Nanoparticle on Differentiation of Human Mesenchymal Stem Cells to Osteoblast
Introduction: IIron oxide nanoparticles (IO NP) have an increasing number of biomedical applications. To date, the potential cytotoxicity of these particles remains an issue of debate. Little is known about the cellular interaction or toxic effects of IO NP on differentiation of stem cells. The aim of the present study was to investigate the possible toxic role of different doses of IO NP in di...
متن کاملthe effects of zinc oxide nanoparticles on differentiation of human mesenchymal stem cells to osteoblast
objective(s):the mesenchymal stem cells (mscs) have been introduced as appropriate cells for tissue engineering and medical applications. some studies have shown that topography of materials especially physical surface characteristics and particles size could enhance adhesion and proliferation of osteoblasts. in the present research, we studied the distinction effect of 30 and 60 μg/ml of zinc ...
متن کاملthe effects of iron oxide nanoparticle on differentiation of human mesenchymal stem cells to osteoblast
introduction: iiron oxide nanoparticles (io np) have an increasing number of biomedical applications. to date, the potential cytotoxicity of these particles remains an issue of debate. little is known about the cellular interaction or toxic effects of io np on differentiation of stem cells. the aim of the present study was to investigate the possible toxic role of different doses of io np in di...
متن کاملSpontaneous in vitro transformation of primary human osteoblast-like cells.
BACKGROUND Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery. MATERIALS AND METHODS The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose. RESULTS AND CON...
متن کاملA serum- and feeder-free technique of culturing human corneal epithelial stem cells on amniotic membrane
PURPOSE To describe a simple technique of cultivating human corneal epithelial stem cells using an Epilife culture medium under serum- and feeder-free conditions. METHODS Cadaveric donor limbal corneal epithelial cells were cultured on denuded amniotic membranes using an explant technique that was free of serum and feeder cells in the Epilife medium containing a growth supplement of defined c...
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ژورنال
عنوان ژورنال: International Journal of Oral and Maxillofacial Surgery
سال: 2017
ISSN: 0901-5027
DOI: 10.1016/j.ijom.2017.02.720